Background: Although various researches have been conducted to improve therapeutic strategies in resolving spinal cord injuries, robust clinical treatment is not yet available. Developing a standard animal model is essential before treatment. Objectives: The present study was performed to introduce a simple, applicable, and minimally invasive approach for access to epidural space in cat. Methods: We used per-cutaneous approach from lumbosacral junction for stainless steel cannula insertion to the epidural space. CT-scan, conventional Magnetic Resonance Imaging, tractography, and behavioral evaluation were used to assess the correct position of cannula and neurological condition of the patient. Results: MRI results showed no significant change in signal intensity index of neural structures under lumbosacral junction. These observations were further supported by tractography, and also behavioral examination during study. Conclusions: We found that per-cutaneous approach from lumbosacral junction is a simple and applicable approach which has no side effects and artifact formation in MRI evaluation.